A Resonance Energy Transfer between Chemiluminescent Donors and Luminescent Quantum-Dots as Acceptors (CRET)

摘要

Fluroescence resonane energy transfer (FRET) is a powerful technique for probing very small changes in the distance between donor and acceptor fluorophores, and is ideal for the sensitive detection of molecular binding events and changes in protein conformation in response to interactions with a particular target colecule or to changes in the solution environment. In recent years, luminescent semiconductor nanocrystals (also called quantum dots, QDs) have been facorably adopted in the FRET-based studies, such as deriving QD-protein-conjugate configuration Qd-protein sensin assemblies, photochromic switching, photodynamic medical therapy, and probin DNA replication and telomerization.