Chimeric LuxR Transcription Factors Rewire Natural Product Regulation

Mukherji Ruchira; Zhang Shuaibing; Chowdhury Somak; Stallforth Pierre

摘要

Abstract > LuxR‐type transcriptional activator proteins frequently regulate the expression of biosynthetic gene clusters (BGCs). With only a fraction of bacterial BGCs being expressed under standard culturing conditions, modulation of LuxRs would provide a powerful approach to activate silent clusters. We show that by exploiting the modular nature of LuxR proteins, it is possible to construct functional chimeric LuxRs, which enables both the rewiring of quorum sensing systems and the activation of silent BGCs. Importantly, our strategy allowed us to identify the novel natural product pseudomonol from a bacterium of the genus Pseudomonas . </abstract> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> <div class="translation abstracttxt"> <span class="zhankaihshouqi fivelineshidden" id="abstract"> <span>机译：</span><Abstract Type =“Main”XML：Lang =“en”> <标题类型=“main”>抽象</ title> > Luxr型转录活化剂蛋白经常调节生物合成基因簇（BGCs）的表达。只有在标准培养条件下表达的一部分细菌BGC，Luxrs的调制将提供一种强大的方法来激活沉默的簇。我们表明，通过利用Luxr蛋白的模块化性，可以构建功能性嵌合Luxrs，这使得频率传感系统的重新安装和静默的BGCS的激活。重要的是，我们的策略使我们可以从属的细菌中识别新的天然产物假单胞菌假单胞菌</ i> 。 </ p> </摘要> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> </div> <div class="record"> <h2 class="all_title" id="enpatent33" >著录项</h2> <ul> <li> <span class="lefttit">来源</span> <div style="width: 86%;vertical-align: text-top;display: inline-block;"> <a href='/journal-foreign-19011/'>《Angewandte Chemie》</a> <b style="margin: 0 2px;">|</b><span>2020年第15期</span><b style="margin: 0 2px;">|</b><span>共4页</span> </div> </li> <li> <div class="author"> <span class="lefttit">作者</span> <p id="fAuthorthree" class="threelineshidden zhankaihshouqi"> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Mukherji Ruchira&option=202" target="_blank" rel="nofollow">Mukherji Ruchira;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Zhang Shuaibing&option=202" target="_blank" rel="nofollow">Zhang Shuaibing;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Chowdhury Somak&option=202" target="_blank" rel="nofollow">Chowdhury Somak;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Stallforth Pierre&option=202" target="_blank" rel="nofollow">Stallforth Pierre;</a> </p> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zkzz" style="display: none;">展开▼</span> </div> </li> <li> <div style="display: flex;"> <span class="lefttit">作者单位</span> <div style="position: relative;margin-left: 3px;max-width: 639px;"> <div class="threelineshidden zhankaihshouqi" id="fOrgthree"> <p>Independent Junior Research Group Chemistry of Microbial CommunicationLeibniz Institute for Natural Product Research and Infection BiologyBeutenbergstrasse 11a 07745 Jena Germany;</p> <p>Independent Junior Research Group Chemistry of Microbial CommunicationLeibniz Institute for Natural Product Research and Infection BiologyBeutenbergstrasse 11a 07745 Jena Germany;</p> <p>Independent Junior Research Group Chemistry of Microbial CommunicationLeibniz Institute for Natural Product Research and Infection BiologyBeutenbergstrasse 11a 07745 Jena Germany;</p> <p>Independent Junior Research Group Chemistry of Microbial CommunicationLeibniz Institute for Natural Product Research and Infection BiologyBeutenbergstrasse 11a 07745 Jena Germany;</p> </div> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zhdw" style="display: none;">展开▼</span> </div> </div> </li> <li > <span class="lefttit">收录信息</span> <span style="width: 86%;vertical-align: text-top;display: inline-block;"></span> </li> <li> <span class="lefttit">原文格式</span> <span>PDF</span> </li> <li> <span class="lefttit">正文语种</span> <span>eng</span> </li> <li> <span class="lefttit">中图分类</span> <span><a href="https://www.zhangqiaokeyan.com/clc/1188.html" title="应用化学">应用化学;</a></span> </li> <li class="antistop"> <span class="lefttit">关键词</span> <p style="width: 86%;vertical-align: text-top;"> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=gene expression&option=203" rel="nofollow">gene expression;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=LuxRs&option=203" rel="nofollow">LuxRs;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=natural products&option=203" rel="nofollow">natural products;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=quorum sensing&option=203" rel="nofollow">quorum sensing;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=transcriptional activation&option=203" rel="nofollow">transcriptional activation;</a> </p> <div class="translation"> 机译：基因表达;Luxrs;天然产品;Quorum感应;转录激活; 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</li> <li> <div> <b>2. </b><a class="enjiyixqcontent" href="/patent-detail/06120101270615.html">对哺乳动物细胞中转基因表达的药剂配体诱导具有增大的灵敏度的嵌合转录因子变异体</a> <b>[P]</b> . <span> 中国专利： CN110291200A </span> <span> . 2019-09-27</span> </div> </li> <li> <div> <b>3. </b><a class="enjiyixqcontent" href="/patent-detail/06130448070388.html">Chimeric transcription factor, useful for detecting specific binding interactions between proteins, is fusion of Pol III transcription factor and DNA-binding domains and includes a conditional mutation</a> <b>[P]</b> . <span> 外国专利：  FR2800077A1 </span> <span> . 2001-04-27</span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：嵌合转录因子可用于检测蛋白质之间的特异性结合相互作用，是Pol III转录因子与DNA结合域的融合，并包括条件突变 </span> </p> </li> <li> <div> <b>4. </b><a class="enjiyixqcontent" href="/patent-detail/06130401679388.html">TARGETED TRANSCRIPTIONAL REGULATION USING SYNTHETIC TRANSCRIPTION FACTORS</a> <b>[P]</b> . <span> 外国专利：  US2020332307A1 </span> <span> . 2020-10-22</span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：使用合成转录因子进行有针对性的转录调控 </span> </p> </li> <li> <div> <b>5. </b><a class="enjiyixqcontent" href="/patent-detail/06130400996682.html">Targeted transcriptional regulation using synthetic transcription factors</a> <b>[P]</b> . <span> 外国专利：  AU2018390957A1 </span> <span> . 2020-07-02</span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：使用合成转录因子的靶向转录调控 </span> </p> </li> </ul> </div> </div> </div> <div class="theme cardcommon" style="overflow: auto;display:none"> <h3 class="all_title" id="enpatent55">相关主题</h3> <ul id="subject"> </ul> </div> </div> </div> </div> <div class="right rightcon"> <div 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