Role of iPLA_2 and store-operated channels in agonist-induced Ca~(2+) influx and constriction in cerebral, mesenteric, and carotid arteries

摘要

Recently, the Ca~(2+)-independent phospholipase A2 (iPLA_2) P (group VI) (2, 30) was found to be a crucial determinant of SOCE activation in isolated aortic SMC, but the role of iPLA_2beta in intact cerebral and other microvessels is unknown. Functional inhibition of iPLA_2 with its suicidal substrate bro-moenol lactone (BEL) or specific antisense oligonucleotides against iPLA_2beta has been shown to produce identical impairment of SOCE in isolated SMC (24, 25), as well as astrocytes (23), keratinocytes (22), skeletal muscle (4), fibroblasts (16), prostate cancer (28), and other cell types (24, 25).