Segmental labeling to study multidomain proteins

摘要

This chapter contains a review of methodologies and recent applications of segmental labeling for NMR structural studies of proteins and protein complexes. Segmental labeling is used to specifically label a segment of protein structure with NMR active nuclei, thus reducing NMR spectral complexity and greatly facilitating structural NMR studies of large multi-domain proteins. It can also be used to introduce a synthetic fragment into a protein structure to study post-translationally modified proteins. Detailed protocols describing segmental labeling techniques are also included.