Evaluation of Haemoglobin and Cytochrome Responses During Forearm Ischaemia Using Multi-wavelength Time Domain NIRS

摘要

We demonstrate the ability of a 16-wavelength time domain near-infrared spectroscopy system to monitor changes in oxy- and dcoxy haemoglobin ([HbO(2)] [HHb]) and the oxidation of cytochrome-c-oxidase ([oxCCO]), during forearm ischaemia. We tested two methods to retrieve the concentration changes. The first uses the measured changes in light attenuation and the modified Beer-Lambert law, and the second uses the absorption and scattering estimated by the measured time-point spread function. The system is able to retrieve the concentration changes with both methods, giving similar results. At the end of forearm ischaemia (t = 5 min), we measured an increase in [HHb] of 16.77 +/- 2.52 and 16.37 +/- 2.33 mu Mol, and a decrease in [HbO(2)] of -6.12 +/- 1.62 and -5.57 +/- 2.02 mu Mol for method 1 and 2, respectively. At that same time, the changes in [oxCCO] were -0.36 +/- 0.33 and -1.40 +/- 1.20 mu Mol, for method 1 and 2, respectively. These small changes in [oxCCO], despite a huge change in haemoglobin, demonstrate the absence of cross-talk and are comparable to previous measurements using broadband NIRS.