Analysis of the genetic diversity for the Juglans hopeiensis cuWwiaxs by FISH-AFLP markers

摘要

The Juglans hopeiensis cultivars were used to study the genetic diversity and parentage relationship in molecular level, providing scientific basis to protect and utilize the germplasm effectively. FISH-AFLP markers were conducted, with 8 primers to analyze the genetic diversity and parentage relationship among 28 Juglans cultivars at genomic DNA level. Totally 1009 AFLP fragments were obtained, in which there were 837 polymorphic bands and 202 characteristic bands. The average percentage of polymorphic bands (PPB) was 82.95% and the genetic similarity coefficient distributed among 0.4446-0.744. The value of effective number of alleles (Ne) ranged from 1.00 to 1.99 with an average of 0.36±0.01; Nei's gene diversity (W) ranged from 0.00 to 0.39 withan average of 0.23±0.01 and Shannon's information index (/) ranged from 0.00 to 0.59 with an average of 0.44±0.02. The /. hopeiensis germplasm had abundant genetic diversity and complex genetic background. Dendrogram of walnut materials origins basedon AFLP data showed that 'shaanxiguanmao' was different, maybe new taxa.