Induction of callus from hypocotyledons and cotyledonary leaves of Ceratonia siliqua.

摘要

Pale yellow callus was formed from segments of seedlings on MS medium+2,4-D (3 mg.L-1)+BAP (3 mg.L-1) after 4 weeks by 58.3%. No callus was induced in case of using MS medium+NAA (0.5 mg.L-1)+Kin (1 mg.L-1) and MS hormone-free. On the other hand, callus was formed on MS medium+BAP (2 mg.L-1) by 71.4%. A large amount of callus formed on MS medium+TDZ (0.5 mg.L-1) and the callus regenerated small leaflets structures when subcultured on MS+TDZ (1 mg.L-1) after 4 weeks. For cotyledonary leaves, callus was formed on MS+NAA (0.5 mg.L-1)+Kin (1 mg.L-1) and MS+2,4 D (3 mg.L-1)+BAP (3 mg.L-1) by 25 and 100%, respectively. No callus was induced in case of using MS hormone-free. Different concentrations of TDZ were also applied, for increasing and multiplication of the callus from cotyledonry leaves.