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首页> 外文期刊>Acta Horticulturae >Analysis of metabolites and expression characteristics of sulfur metabolism related genes in 'Nanou' garlic and common garlics based on LC-MS
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Analysis of metabolites and expression characteristics of sulfur metabolism related genes in 'Nanou' garlic and common garlics based on LC-MS

机译:基于LC-MS的南路大蒜和普通大蒜硫代谢相关基因代谢产物及表达特征分析

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摘要

In this study, LC-MS (liquid chromatography-mass spectrometry) was used to detect the differences in the whole metabolism group and the difference in the content of partial sulfur metabolites in 'Nanou' garlic and common garlic. Meanwhile, 6 genes related to the sulfur metabolism in garlic were analyzed by qRT-pcr. The results showed that 'Nanou' was significantly different from the common garlic in the metabolic groups and the content of sulfur metabolites. The content of allicin and alliin in 'Nanou' was significantly lower than that of common garlic, and the difference in the expression of genes encoding related enzymes was in accordance with the content of sulfur metabolites.
机译:在该研究中,使用LC-MS(液相色谱 - 质谱)来检测整个代谢组的差异,以及南路大蒜和普通大蒜中部分硫代谢物含量的差异。 同时,通过QRT-PCR分析了与大蒜中硫代谢相关的6个基因。 结果表明,“南路”与代谢组中的普通大蒜和硫代谢物含量显着不同。 “南路”的含量和Alliin的含量显着低于普通大蒜,编码相关酶的基因表达的差异是符合硫代谢物的含量。

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