Cryopreservation technique for lettuce, Chinese cabbage, flowering white cabbage, Chinese kale and pak choi seeds by encapsulation-dehydration

摘要

The Plant Genetic Conservation Project under the Royal Initiation of Her Royal Highness Princess Maha Chakri Sirindhorn (RSPG) has collaborated with the DOA Genebank (Genebank Research and Development Group, Biotechnology Research and Development Office, Department of Agriculture), to study the cryopreservation techniques of lettuce, Chinese cabbage, flowering white cabbage, Chinese kale and pak choi seeds by encapsulation-dehydration method. It was found that the seeds which were not plunged into liquid nitrogen (LN) had 100% viability in every experiment. It appeared that seeds could germinate to be normal seedlings on MS medium after 14 d. The duration time for loading solution (0.8 M sucrose + 1 M glycerol) was 10 and 20 min and the duration time for silica gel was 14 and 21 h and did not damage the seeds. Cryopreserved seeds showed normal seedlings on MS medium at 14 d. It was found that lettuce, Chinese kale and pak choi had 100% survival. For Chinese cabbage and flowering white cabbage, theyhad 60-90.9 and 25-91.60% survival, respectively, and were able to grow in the field conditions. Therefore, the conservation of these seeds in LN by encapsulation-dehydration is the optional for the conservation of these vegetable seeds.