Strategies for maintaining and increasing throughput of in vitro cultures of sugarcane

摘要

Simple in vitro protocols were developed or improved for in vitro manipulations of sugarcane [Saccharum species hybrids) to free up resources, deal with back-logs and increase throughput in research and commercial laboratories. These include: (1) in vitro germination of field-derived axillary buds for meristem isolation; (2) a 'common' medium for rapid shoot multiplication resulting from single genetic modification events; (3) shoot storage for 12 months; (4) 'holding-back' in vitro plantlets beforeacclimation. The novel strategies have proven reliable and effective in managing changing demands on resources and priorities.