Establishment of photomixotrophic cultures for high-scale micropropagation by temporary immersion bioreactors (TIBs) in plant commercial species

摘要

The optimization of photomixotrophic conditions (increase of both light intensity and the C02 atmosphere inside the culture flask) improves plant quality and can lead to greater efficiency during the acclimatization phase. For this case, temporary immersion bioreactors (TIBs) installed in controlled environment characterized by C02 enrichment, higher luminosity, and sucrose-reduced medium might be considered as priming treatments, support on the TIB plasticity as an integrative tool for a high-scalemicropropagation. Examples in berries as commercial blueberries (Vaccinium corymbosum), raspberries (Rubus ideaus) and a forest plant (dendroenergetic poplars - Populus spp.) should be presented related to an increase in the efficiency of plants' survival and their adaptability to field conditions.