The effect of calcitonin gene -related peptide functionalized TiO 2 nanotubes on osteoblast and osteoclast differentiation in vitro

摘要

Osteoporosis is a disease that causes an increased risk of bone fracture. Our goal was to enhance the bioactivity and anti-osteoporotic properties of titanium implants by immobilizing calcitonin gene -related peptide (CGRP) onto TiO 2 nanotubes through polydopamine film. The response behaviors of osteoblasts and osteoclasts cultured onto unmodified and modified TiO 2 nanotube surfaces were evaluated in vitro . Alkaline phosphatase (ALP) activity, mineralization potential and osteogenic marker gene expression (Runx2, ALP, Col I, OPN, OPG and RANKL) indicated that osteoblast differentiation was greater on CGRP modified TiO 2 nanotubes than those on other substrate surfaces. Additionally, tartrate -resistant acid phosphatase (TRAP) and osteoclastic-related gene expression (VATPase, MMP9, TRAP and CTSK) showed that osteoclast differentiation was lowest on CGRP modified TiO 2 nanotubes. These results indicate that CGRP functionalized TiO 2 nanotubes enhance osteoblast differentiation as well as suppress osteoclast resorption. This study provides design modifications of titanium applied as osteoporotic bone implants.