首页> 外文期刊>Basic & clinical pharmacology & toxicology. >Differentiated mitochondrial function in mouse 3T3 fibroblasts and human epithelial or endothelial cells in response to chemical exposure
【24h】

Differentiated mitochondrial function in mouse 3T3 fibroblasts and human epithelial or endothelial cells in response to chemical exposure

机译:在小鼠3T3成纤维细胞和人上皮或内皮细胞中的分化型线粒体功能响应于化学暴露

获取原文
获取原文并翻译 | 示例
       

摘要

Mouse 3T3 fibroblasts are commonly used for in vitro toxicity testing; however, their sensitivity to stimuli is not well defined. To assess the sensitivity of the 3T3 cell line, the study compared the changes in mitochondrial membrane potential (MMP) occurring after exposure to eight chemicals known to demonstrate pro-apoptotic activity (glycerol, isopropanol, ethanol, paracetamol, propranolol, cobalt chloride, formaldehyde and atropine). Five cell lines were used as follows: mouse 3T3 fibroblasts, human epithelial cells (A549, Caco-2 and HepG2) and human endothelial cells (HMEC-1). Cell sensitivity was assessed based on the total area under and over the dose-response curves (AUOC) in relation to baselines. The 3T3 fibroblasts had the highest AUOC values and were the most sensitive to the action of all the examined chemicals, with the exception of formaldehyde. Significant changes in MMP between the 3T3 cell line and other cells were observed after cell treatment with atropine (A549, Caco-2 or HMEC-1 cells vs 3T3 cells, P 0.05), propranolol (A549 vs 3T3 cells, P 0.01; HepG2 vs 3T3 cells, P 0.05), cobalt chloride (A549 cells vs 3T3 cells, P 0.01) or ethanol (HMEC-1 vs 3T3, P 0.05). Formaldehyde appeared the most toxic compound for Caco-2 cells (Caco-2 vs 3T3 cells, P 0.05). The surface areas (AUOC) calculated for each other chemical and obtained for HepG2, Caco-2, A549 and HMEC-1 did not differ significantly between cell lines. We postulate that mouse 3T3 fibroblasts demonstrate significantly higher relative sensitivity to many agents with toxic potential.
机译:小鼠3T3成纤维细胞通常用于体外毒性测试;然而,它们对刺激的敏感性并不明确。为了评估3T3细胞系的敏感性,研究比较了暴露于八种化学物质后发生的线粒体膜电位(MMP)的变化,该化学物质已知的促凋亡活性(甘油,异丙醇,乙醇,扑热氨基酚,丙醇醇,氯化钴,甲醛和阿托品)。使用五种细胞系如下:小鼠3T3成纤维细胞,人上皮细胞(A549,Caco-2和HepG2)和人内皮细胞(HMEC-1)。基于与基线的剂量 - 反应曲线(AuOC)下的总面积和过度的总面积评估细胞敏感性。除甲醛除外,3T3成纤维细胞具有最高的AuOC值,对所有检查的化学品的作用最敏感。在用阿托品(A549,CaCO-2或HMEC-1细胞Vs 3T3细胞,P <0.05),普萘洛尔(A549 Vs 3T3细胞,P&lt3细胞,P <0.05),观察到3T3细胞系和其他细胞之间的MMP的显着变化。(A549 VS 3T3细胞,P& 0.01; hepg2 vs 3t3细胞,p <0.05),氯化钴(A549细胞与3T3细胞,P <0.01)或乙醇(HMEC-1 Vs 3T3,P <0.05)。甲醛出现了Caco-2细胞最有毒的化合物(Caco-2 Vs 3T3细胞,P <0.05)。为彼此化学品计算并获得HepG2,Caco-2,A549和HMEC-1的表面积(AuOc)在细胞系之间没有显着差异。我们假设小鼠3T3成纤维细胞对许多具有毒性潜力的同学表现出显着更高的相对敏感性。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱:kefu@zhangqiaokeyan.com

京公网安备:11010802029741号 ICP备案号:京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有
  • 客服微信

  • 服务号