Development of a Vivid FRET System Based on a Highly Emissive dG–dC Analogue Pair

摘要

A new type of F?rster Resonance Energy Transfer (FRET) system using highly emissive isomorphic nucleobase analogues is reported. The FRET pair consists of 2-aminothieno[ 3,4-d]pyrimidine G-mimic deoxyribonucleoside (~(th)dG) as an energy donor and 1,3-diaza-2-oxophenothiazine (tC) as an energy acceptor. The distance and orientation between donor and acceptor was controlled by systematic incorporation of ~(th)dG and tC into DNA sequences to investigate the FRET efficiencies. This is the first Watson–Crick base-pairable FRET pair to produce vivid colors. In addition, this nucleic acid-based FRET pair was used to monitor DNA conformation and achieved visualization of the B–Z transition.