Selective protein quantification for preparative chromatography using variable pathlength UV/Vis spectroscopy and partial least squares regression

摘要

Graphical abstractDisplay OmittedHighlights?PAT tool for monitoring of preparative chromatographic steps.?In-line UV/Vis spectral measurement at protein concentrations up to 75?g/l.?Selective protein quantification by partial least squares regression (PLS).?Control of purification steps by calculating pool purities with PLS models.AbstractIn preparative protein chromatography, broad dynamic ranges of protein concentrations as well as co-elution of product and impurities are common. Despite being the standard in biopharmaceutical production, monitoring of preparative chromatography is generally limited to surro