Effect of Vitrification on Functional Morphology and Viability of the Ovarian Tissue

Yuzhakov V. V.; Malinova I. V.; Kiseleva M. V.; Fomina N. K.; Bandurko L. N.; Komarova E. V.; Sevankaeva L. E.; Ingel I. E.; Yakovleva N. D.; Kaprin A. D.

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Effect of Vitrification on Functional Morphology and Viability of the Ovarian Tissue

机译：玻璃化对卵巢组织功能形态和活力的影响

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The results of a complex morphofunctional study with markers of proliferation (PCNA and Ki-67), angiogenesis (CD31 and CD34), and structural integrity of mesenchymal cells (vimentin) suggest that the pool of primordial follicles was well preserved in vitrified ovarian tissue and that viability of its cellular components can be rapidly restored during incubation at 37A degrees C within 4 h after thawing.

机译：具有增殖的标志物（PCNA和Ki-67），血管生成（CD31和CD34）和间充质细胞（Vimentin）的结构完整性的结果表明，原始卵泡的池在玻璃化卵巢组织中得到了很好的保存可以在解冻后4小时内在37A℃的温育期间快速恢复其细胞组分的活力。

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《Bulletin of experimental biology and medicine》 |2018年第4期|共6页
作者
Yuzhakov V. V.; Malinova I. V.; Kiseleva M. V.; Fomina N. K.; Bandurko L. N.; Komarova E. V.; Sevankaeva L. E.; Ingel I. E.; Yakovleva N. D.; Kaprin A. D.;
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作者单位

Minist Hlth Russian Federat A Tsyb Med Radiol Res Ctr Branch Natl Med Res Radiol Ctr Obninsk;

Minist Hlth Russian Federat A Tsyb Med Radiol Res Ctr Branch Natl Med Res Radiol Ctr Obninsk;

Minist Hlth Russian Federat A Tsyb Med Radiol Res Ctr Branch Natl Med Res Radiol Ctr Obninsk;

Minist Hlth Russian Federat A Tsyb Med Radiol Res Ctr Branch Natl Med Res Radiol Ctr Obninsk;

Minist Hlth Russian Federat A Tsyb Med Radiol Res Ctr Branch Natl Med Res Radiol Ctr Obninsk;

Minist Hlth Russian Federat A Tsyb Med Radiol Res Ctr Branch Natl Med Res Radiol Ctr Obninsk;

Minist Hlth Russian Federat A Tsyb Med Radiol Res Ctr Branch Natl Med Res Radiol Ctr Obninsk;

Minist Hlth Russian Federat A Tsyb Med Radiol Res Ctr Branch Natl Med Res Radiol Ctr Obninsk;

Minist Hlth Russian Federat A Tsyb Med Radiol Res Ctr Branch Natl Med Res Radiol Ctr Obninsk;

Minist Hlth Russian Federat A Tsyb Med Radiol Res Ctr Branch Natl Med Res Radiol Ctr Obninsk;

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正文语种 eng
中图分类基础医学;
关键词
ovarian tissue; follicles; vitrification; immunohistochemistry;

机译：卵巢组织;卵泡;玻璃化;免疫组化;

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专利

1. Effect of Vitrification on Functional Morphology and Viability of the Ovarian Tissue [J] . Yuzhakov V. V., Malinova I. V., Kiseleva M. V., Bulletin of experimental biology and medicine . 2018,第4期

机译：玻璃化对卵巢组织功能形态和活力的影响
2. Morphologic, viability and ultrastructural analysis of vitrified sheep preantral follicles enclosed in ovarian tissue. [J] . Lunardi F. O., Araujo V. R., Faustino L. R., Small Ruminant Research . 2012,第2a3期

机译：卵巢组织中玻璃化的绵羊窦前卵泡的形态，生存力和超微结构分析。
3. Morphological, ultrastructural and functional imaging of frozen/thawed and vitrified/warmed human ovarian tissue retrieved from oncological patients [J] . Fabbri R., Vicenti R., Macciocca M., Human Reproduction . 2016,第8期

机译：取自肿瘤患者的冷冻/融化和玻璃化/变暖的人类卵巢组织的形态学，超微结构和功能成像
4. The Profile of Ovarian Tissue After Vitrification Using Ethylene Glycol (EG) and Longan Honey in Female Sprague-Dawley Rats (Rattus norvegicus L.) [C] . I. Muhiardi, N. Anita, A. Abinawanto, International Symposium on Current Progress in Mathematics and Sciences . 2019

机译：使用乙二醇（例如）乙二醇和龙橙蜂蜜在雌性Sprague-dawley大鼠（Rattus Norvegicus L.）之后卵巢组织概况
5. Assessment of viability of cryopreserved ovarian tissue. [D] . Nubani, Reem Yaser. 2005

机译：评估冷冻保存的卵巢组织的活力。
6. Morphological and functional preservation of pre-antral follicles after vitrification of macaque ovarian tissue in a closed system [O] . A. Y. Ting, R. R. Yeoman, J. R. Campos, -1

机译：在封闭系统中猕猴卵巢组织玻璃化后窦前卵泡的形态和功能保留
7. Morphologic, viability and ultrastructural analysis of vitrified sheep preantral follicles enclosed in ovarian tissue [O] . Lunardi Franciele Osmarini, Araújo Valdevane Rocha, Faustino Luciana Rocha, 2012

机译：绵羊卵囊包裹在卵巢组织中的形态，生存能力和超微结构分析
8. Identification and Functional Characterization of Somatic Mutations in Human MicroRNAs and their Responsive Elements in Target Genes in Ovarian Tumor Tissues [R] . Zhao, H. 2009

机译：人类微小RNa中体细胞突变的鉴定和功能表征及其在卵巢肿瘤组织靶基因中的反应元件

Effect of Vitrification on Functional Morphology and Viability of the Ovarian Tissue

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