Inhibitory Activity of Scutellaria baicalensis Flavonoids against Tick-Borne Encephalitis Virus

摘要

We studied virus-inhibiting activity of Baikal skullcap (Scutellaria baicalensis) flavonoids against tick-borne encephalitis virus using various model schemes. The half-maximum cytotoxic concentration (CC50) for the plant extract was found (363.9 +/- 58.6 mu g/ml). Based on the CC50 and IC50, selective index (SI) was calculated for viricidal (53.4), preventive (50.5), and direct antiviral actions (39.1) and for-intracellular replication of the virus (40.4). Suppression of virus reproduction >= 2.0 lg TCID50 was observed at extract concentration >= 5 mu g/ml (viricidal effect), >= 11.2 mu g/ml (preventive and direct antiviral effects), and >= 9 mu g/ml (intracellular replication). Flavonoids of Baikal skullcap extract produced an in vitro inhibitory effect on tick-borne encephalitis virus due to their direct viricidal activity and direct inhibition of adsorption and intracellular replication of tick-borne encephalitis virus, which determines their value as highly effective antiviral drugs.