首页> 外文期刊>Bulletin of experimental biology and medicine >A Novel Express Method to Determine Activity of Antitumor Enzyme L-Lysine-alpha-Oxidase of Trichoderma harzianum Rifai F-180
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A Novel Express Method to Determine Activity of Antitumor Enzyme L-Lysine-alpha-Oxidase of Trichoderma harzianum Rifai F-180

机译:一种测定Trichoderma Harzianum Rifai F-180抗肿瘤酶L-赖氨酸-α-氧化酶活性的新型表达方法

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摘要

A novel express method is developed to determine activity of antitumor enzyme L-lysine-alpha-oxidase obtained by culturing Trichoderma harzianum Rifai F-180 fungus. The carcinogenic reagent ortho-dianisidine-hydrochloride was replaced in the reaction medium with environmentally friendly reagents of the chromogenic mixture that included tetramethylbenzidine. This method improved precision and sensitivity of ELISA by 10 and 40 times, respectively. In addition, it could detect activity of L-lysine-alpha-oxidase not only in the producer strains with a pronounced activity of this enzyme, but also in the strains where this activity has not been previously determined.
机译:开发了一种新型表达方法以确定通过培养Trichoderma Harzianum Rifai F-180真菌获得的抗肿瘤酶L-赖氨酸-α-氧化酶的活性。 在具有包含四甲基苯甲酸的发色混合物的环保混合物的反应介质中,在反应介质中取代了致癌试剂邻二氨基乙胺 - 盐酸盐。 该方法分别提高了ELISA的精度和敏感性10至40次。 此外,它还可以检测L-赖氨酸 - α-氧化酶的活性,不仅在生产者菌株中具有该酶的显着活性,还可以在所述活性尚未确定该活性的菌株中。

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