A fully automated microfluidic-based electrochemical sensor for real-time bacteria detection

摘要

AbstractA fully automated microfluidic-based electrochemical biosensor was designed and manufactured for pathogen detection. The quantification ofEscherichia coliwas investigated with standard and nanomaterial amplified immunoassays in the concentration ranges of 0.99 × 1043.98 × 109cfu mL?1and 103.97 × 107cfu mL?1which resulted in detection limits of 1.99 × 104cfu mL?1and 50 cfu mL?1, respectively. The developed methodology was then applied forE. coliquantification in water samples using nanomaterial modified assay. Same detection limit forE. coliwas achieved for real sample analysis with a little decrease on the sensor signal. Cross-reactivity studies were conducted by testingShigella,Salmonellaspp.,Salmonella typhimuriumandStaphylococcus aureusonE. colispecific antibody surface that confirmed the high specificity of the developed immunoassays. The sensor surface could be regenerated multiple times which significantly reduces the cost of the system. Our custom-designed biosensor is capable of detecting bacteria with high sensitivity and specificity, and can serve as a promising tool for pathogen detection.展开▼