Refinement of intratracheal dosing techniques in rats

摘要

Intratracheal (IT) dosing is used in drug discovery as a method of delivering drugs directly to the pulmonary system allowing the determination of the efficacy or pharmokinetics of compounds, IT dosing ensures delivery of 100% of the dose to the lungsand does not require any complex optimisation of the dose formulation, as is required for administration via inhaled routes eg. Buxco system. IT dosing is used as a surrogate for inhaled administration in man. To improve percentage of accurately delivered doses to the lungs. Success rate for a trained technician using the previous standard technique is normally less than 95%. However, accuracy may be lower during training and pharmokinetics profiles become hard to determine.