Insight into the microbial community and its succession of a coupling anaerobic-aerobic biofilm on semi-suspended bio-carriers

摘要

Highlights?A coupling anaerobic-aerobic biofilm was established on semi-suspended bio-carriers.?The coupling biofilm created suitable multi-habitats for diverse microorganisms.?The coupling biofilm stimulated the simultaneous removal of organics and nutrients.?The microbial community on bio-carrier was dominated by functional microorganisms.AbstractThis work aims at establishing a coupling anaerobic-aerobic biofilm within a single bioreactor and revealing its microbial community and succession. By using a semi-suspended bio-carrier fabricated with 3D printing technique, an obvious DO gradient was gradually created within the biofilm, which demonstrated that a coupling anaerobic-aerobic biofilm was successfully established on the surface of bio-carriers. The results of metagenomic analysis revealed that the microbial community on the bio-carriers experienced a continuous succession in its structure and dominant spe