Selective separation of biomolecules by reversed micelles-extraction of proteins and nucleic acids-

摘要

Reversed micelles,which are nanostructural moelcular assemblies formed by surfactant molecules in an organic solvent,are a promising separation tool for biomolecules such as amino acids,proteins and nucleic acids.In the separation by reversed micelles,biomolecules transfer from an aqueous soution into a reversed micellar phase through the interaction between surfactant moelcules and a target biomolecule:e.g.electrostatic interaction has been found to play an important role in the degree of extraction,and the design of reversed micelles often decides the success of separtion efficiency.In this article,we reviewed the recent investigation concerning the reversed micellar exraction techniques for biomolecules;mainly proteins and deoxyribonucleic acids (DNAs).We also describe the transfer mechanism of proteins through the protein-surfactant complexes at the liquid-liquid interface.DNAs were first demonstrated to be transferred from an aqueous solution to the reversed micellar phase by using a cationic surfactant.Furthermore,we introduce recent novel application of reversed micelles to the separation of biomolecules.