Genetic transformation of pea (Pisum sativum L.) by electroporation of protoplasts

Yu. V. Simonenko; N. V. Kuchuk

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Genetic transformation of pea (Pisum sativum L.) by electroporation of protoplasts

机译：原生质体电镀豌豆（Pisum Sativum L.）的遗传转化

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Transgenic clones and plants of pea (Pisum sativum L. ) containing the L-phosphinothricin-resistance gene have been produced by electroporation of mesophyll protoplasts. Phosphinothrtcin-resistance gene (synthetic bar gene) was transferred to plant protoplasts using pUC35Sbar plasmid under the 35S-promotor of CaMV. The fact of gene integration into calluses and regenerated plants was confirmed by DNA analysis.

机译：通过叶片原生质体的电穿孔制备了含有L-膦腈抗基因的豌豆（Pisum Sativum L.）的转基因克隆和植物。使用PUC35SBAR质粒在CAMV的35s-启动器下将磷噻吩抗性基因（合成杆基因）转移到植物原生质体中。通过DNA分析证实了基因集成成愈伤组织和再生植物的事实。

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《Физиология и биохимия культурных растений: Науч.- теорет. журн.》 |1999年第3期|共5页
作者
Yu. V. Simonenko; N. V. Kuchuk;
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作者单位

Institute of Cell Biology and Genetic Engineering National Academy of Sciences of Ukraine 148 Acad. Zabolotny St. Kyiv 252143 Ukraine;

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原文格式 PDF
正文语种 rus
中图分类植物生理学;
关键词
pea (Pisum sativum L.); transformation; electroporation; phosphinothricin;

机译：豌豆（Pisum sativum l。）;转化;电穿孔;膦腈;

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1. Genetic transformation of pea (Pisum sativum L.) by electroporation of protoplasts [J] . Yu. V. Simonenko, N. V. Kuchuk Физиология и биохимия культурных растений: Науч.- теорет. журн. . 1999,第3期

机译：原生质体电穿孔对豌豆（Pisum sativum L.）的遗传转化
2. Processing Peas (Pisum sativum L.).Part 4: Blanching Process Optimization Pea (Pisum sativum L.) Cultivares Sure?a and Andina based on the Inactivation of Peroxidase [J] . Pinchao Yamid A, Andrade Johana C, Osorio Oswaldo Informacion Tecnologica . 2016,第4期

机译：加工豌豆（Pisum sativum L.）。第4部分：基于过氧化物酶失活，优化豌豆（Pisum sativum L.）栽培品种Sure？a和Andina
3. Processing Peas (Pisum sativum L.) Part 5: Development of an Edible Coating Compound for the Conservation of Pea (Pisum sativum L.) [J] . Andrade Johana C, Pinchao Yamid A, Osorio Oswaldo, Informacion Tecnologica . 2016,第5期

机译：加工豌豆（Pisum sativum L.）第5部分：开发保护豌豆（Pisum sativum L.）的可食用包衣化合物
4. Tissue Culture and Protoplast Fusion of Cool-Season Pulses: Pea (Pisum sativum L.) and Chickpea {Cicer arietinum L.) Short Title: Tissue Culture and Protoplast Fusion of Pulses [C] . E. C. K. Pang, J. S. Croser, K. T. Imberger, International Food Legumes Research Conference . 2000

机译：凉爽季脉冲组织培养和原生质体融合：豌豆（Pisum sativum L.）和鹰嘴豆（Cicer arietinum L.）短冠名：组织培养和脉冲原生质体融合
5. Anti-nutritional factors in field pea (Pisum sativum L.) and grass pea (Lathyrus sativus L.). [D] . Wang, Xiaofang. 1997

机译：豌豆（Pisum sativum L.）和豌豆（Lathyrus sativus L.）中的抗营养因子。
6. Treatment of pea (Pisum sativum L.) protoplasts with DNA-damaging agents induces a 39-kilodalton chloroplast protein immunologically related to Escherichia coli RecA. [O] . H Cerutti, H Z Ibrahim, A T Jagendorf 1993

机译：用DNA破坏剂处理豌豆（Pisum sativum L.）原生质体会诱导与大肠杆菌RecA免疫相关的39千达尔顿叶绿体蛋白。
7. Consumo médio e digestibilidade aparente dos nutrientes do feno de aveia (Avena sativa L.) e ervilha (Pisum sativum L.) em ovinos submetidos a dois regimes alimentares Oat hay (Avena sativa L.) and pea (Pisum sativum L.) nutrients digestibility and nutrients average intake by sheep under two feeding systems [O] . Ivone Yurika Mizubuti, Carlos Armênio Khatounian, Edson Luis de Azambuja Ribeiro, 2002

机译：两种饮食下的绵羊燕麦干草（Avena sativa L.）和豌豆（Pisum sativum L.）养分的平均消耗和表观消化率燕麦干草（Avena sativa L.）和豌豆（Pisum sativum L.）养分消化率两种饲养方式下绵羊的平均养分和养分摄入量

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Genetic transformation of pea (Pisum sativum L.) by electroporation of protoplasts

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