Production of Fusarium solani f.sp.pisi cutinase in Fusarium venenatum A3/5

摘要

Fusarium venenatum A3/5 was transformed using the Aspergillus niger expression plas-mid,pIGF,in which the coding sequence for the F.solani f.sp.pisi cutinase gene had been inserted in frame,with a KEX2 cleavage site,with the truncated A.niger glucoamylase gene under control of the A.niger glucoamylase promoter.The transformant produced up to 21 U cutinase 1~(-1) in minimal medium containing glucose or starch as the primary carbon source.Glucoamylase (165 U 1~(-1) or 8 mg 1~(-1)) was also produced.Both the transformant and the parent strain produced cutinase in medium containing cutin.