Characterization of a Cell Invasion Assay for High-Content Screening Using a Laser Microplate Cytometer

摘要

Cancer invasion from a primary tumor site is one of the critical factors in disease prognosis,~1 and together with metastasis is the major cause of morbidity and mortality. The molecular and cellular processes that govern tumor invasion and metastasis are complex and involve multiple pathogenic steps, including changes to the adhesive and migratory capabilities of tumor cells and the tumor microenvironment, and invasion of the surrounding extracellular matrix (ECM).~(2,3) In order to delineate these complex and precise mechanisms, in vitro models are needed that reliably and accurately recreate the metastatic process.