Evaluation of Surface-Enhanced Laser Desorption Time-of-Flight Mass Spectroscopy in the Development of Biomarkers of Occupational Acrylamide Exposure

摘要

Surface-enhanced laser desorp tion time-of-flight mass spec troscopy (SELDI-TOF-MS) is a bioanalytical technique used for the rapid examination of intact protein or protein mixtures to exploit the bio chemical or biophysical characteristics of intact molecules to separate a com plex protein mixture or isolate specific protein classes. Surface-enhanced laser desorption time-of-flight mass spectros copy allows for rapid examination of protein components of body fluids or cell lysates without extensive extraction or preparative measures. The levels and composition of proteins found in blood and urine may change after exposure to toxic agents.~(1￡?2) Such potential changes make the proteins found in these eas ily obtained fluids a desirable source of new or altered proteins that indicate toxic exposure. This article describes the use of SELDI-TOF to examine urinary proteins or hemoglobin present in erythrocyte lysates.