ELISA-based determination of natural antibodies to endothelial NO-synthase in human blood and tear

摘要

Research of natural antibodies (NAA) is a new trend in immunohistochemistry. NAA shape up, in biological fluids, as normal human endogenous low- and high-weight substances, e.g. angiotensin 2, bradykinin and others, and play the regulatory role in homeostasis. Endothelial NO-synthase (e-NOS) is a key enzyme in the path of NO shaping. An e-NOS alteration is important in the pathogenesis of diabetic microangiopathy including retinopathy. We determined, within our case study, the level of NAA to e-NOS in blood and tears of patients with diabetic retinopathy and in donors. The level of NAA to e-NOS was found to be twice higher in tears versus blood in all groups of patients. It depended on specific features and complications of diabetes mellitus. sleze cheloveka metodom immunofermentnogo analiza.