The results of implementation of the external quality assessment program for tests for HBsAg by 'Vector-Best' in 68 laboratories of Russia

摘要

The external quality assessment program (EQAP) for tests of blood samples for HBsAg has been developed, which comprises a control panel of blood samples containing various levels of HBsAg; a control set for HBsAg detection; test assignments including a number of questions on the theory and practice of enzyme immunoassay; accompanying documents stipulating the conditions and procedure of participation in EQAP. Examination of the association of the results of tests using control panels with the answers given by the staff of 68 laboratories responding the questions of test assignments has revealed that the correction determination of positive control samples is significantly associated with the use of disposable vessels for the solutions of the conjugate, TMB, buffer for conjugate dissolution, CFR, with that of the boiling stage for the treatment of non-expendable tips, or with the use of disposable ones.