Sugarcane juice as an alternative carbon source for in vitro culture of plantains and bananas.

摘要

Suitability of sugarcane juice for the in vitro cultivation of bananas and plantains was investigated. 2 concentrations of sugar cane juice (5 and 10%) and 30 g l--1 laboratory sucrose were tested. Explants prepared from field grown sword suckers were cultured at 26 degrees C, with a 16 h photoperiod, light intensity of 3000 lux and 60% RH. Plants that were cultured on 5% sugarcane juice had higher average fresh wt. (16.6 g per plant) than those cultured on 30 g l--1 sucrose and 10% sugarcane juice, which had fresh wt. of 15.6 and 11.0 g per plant, respectively. Plants cultured on 5% sugarcane juice demonstrated significantly greater shoot height and number of shoots per plant than those cultured on 30 g l--1 sucrose. Results suggest that 5% sugarcane juice can be used as a substitute for laboratory grade sucrose for the in vitro propagation of Musa sp.