Optimizing the Western Blotting Procedure: Solutions for Faster Western Blots

摘要

Western blotting is a foundation technique for protein chemistry. The basic technique-transferring size-separated protein bands from a gel onto a membrane and visualizing with the use of antibodies-has been in existence for decades, and the protocols have changed very little. However, incremental improvements in materials, equipment, and reagents have made the technique continually more reliable and more quantitative over the years. In addition, technology advances are making the Western blotting procedure faster than ever, shaving time off the preparation, run, and analysis stages of the process. Solutions for faster Western blotting include faster reagents, faster transfer equipment, and even whole systems built from the ground up for a faster overall procedure, from sample prep to results.