The Use of Gel Permeation Chromatography for the Cleanup of Samples in the Analytical Laboratory

摘要

Gel permeation chromatography(GPC) is a subset of a very com-mon technique called size exclusionchromatography (SEC), which involvesthe separation of molecules by their hydro-dynamic volume in solution, commonlyreferred to as the molecular size.' Thisshould not be confused with the molecularweight. When the sample is passed througha porous stationary phase, the molecularsize of the solvated molecules determinestheir ability to enter the pores of the resin.Thus GPC is the separation of moleculesaccording to molecular size when using anorganic solvent as the mobile phase (Figure1). The vast majority of laboratories thatuse this technique use it as an analyticaltool to measure the distribution within thesample. Commonly, the analyzes of interestare a protein or a polymer.'