Crystallization and preliminary crystallographic studies of the recombinant antitumour lectin from the edible mushroom Agrocybe aegerita

摘要

The antitumour lectin from Agrocybe aegerita, named AAL, shows strong inhibition effects on human and mouse tumour cells via apoptosis induction activity. Recombinant AAL (rAAL) has been expressed and purified. Both rAAL and rAAL-lactose complex have been crystallized and their X-ray diffraction data were collected to resolutions of 1.9 angstrom and 1.6 angstrom, respectively. Both crystals belong to space group P2(1) with unit cell parameters a=53.20 angstrom, b=66.01 angstrom, c = 57.86 angstrom, beta = 109.38 and a = 53.38 angstrom, b = 66.29 angstrom, c = 58.02 angstrom , beta = 109.03, respectively. (c) 2005 Elsevier B.V. All rights reserved.