Safety Evaluation of a Food Containing Salmon Nasal Cartilage-derived Proteoglycan (Proteoglycan F) A Single-dose Toxicity Study in Rats

摘要

Proteoglycans (PG), as well as collagen and hyaluronic acid, are contained in large amounts in connective tissues, such as cartilage and skin, as important components of the extracellular matrix. Recently, a method for extracting PG from salmon nasal cartilage using acetic acid solution was developed, and salmon nasal cartilage-derived proteoglycan (SPG) has been reported to have anti-inflammatory and joint-protective effects. However, no single-dose toxicity studies of SPG have been reported yet. Therefore, we conducted a single-dose toxicity study by forced oral administration of a food containing salmon nasal cartilage-derived proteoglycan (Proteoglycan F) (5000 mg/kg body weight, 1000 mg/kg body weight as SPG) to Wistar-Hannover/Rcc rats- the animals did not die and showed no loss of body weight during the study period, and gross pathological examination revealed no abnormalities after the study period. The results suggested that the lethal dose of proteoglycan F is higher than 5000 mg/kg body weight in male and female rats.