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首页> 外文期刊>Журнал микробиологии, эпидемиологии и иммунобиологии: Двухмес. науч.-практ. журн. >Development and approbation of test systems for the determination of herpes simplex virus and cytomegalovirus antigens by the method of lanthanide immunofluorescent assay
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Development and approbation of test systems for the determination of herpes simplex virus and cytomegalovirus antigens by the method of lanthanide immunofluorescent assay

机译:用镧系元素免疫荧光测定法测定单纯疱疹病毒和细胞瘤病毒抗原试验系统的开发和认可

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摘要

On the basis of the lanthanide immunofluorescent assay (UFA) test systems for the determination of herpes simplex virus (HSV-LIFA) and cytomegalovirus (CMV-UFA) antigens have been developed. The test system HSV-LIFA includes the sandwich of monoclonal antibodies (McAb) HSV A.3.3. or B-11 to type 2 HSV, strain BH; the test system CMV-UFA includes the sandwich of rabbit McAb to CMV, strain AD 169. The approbation of the test systems has revealed that they insure the specific detection of HSV and CMV antigens in clinical specimens (urine, blood, liquor, saliva), the UFA results well correlating with the data on the isolation of viruses in cell cultures, with the results obtained by other diagnostic methods and with clinical manifestations of diseases. UFA has been shown to be more sensitive than the enzyme immunoassay.
机译:基于镧系元素免疫荧光测定(UFA)测试系统,用于测定疱疹病毒(HSV-LIFA)和缩细胞病毒(CMV-UFA)抗原。 测试系统HSV-LIFA包括单克隆抗体(MCAB)HSV A.3.3的三明治。 或B-11型2 HSV,菌株BH; 测试系统CMV-UFA包括兔MCAB的三明治至CMV,菌株AD 169.测试系统的认可揭示他们确保在临床标本(尿液,血液,液体,唾液)中的HSV和CMV抗原的特异性检测 ,UFA结果与细胞培养物中病毒分离的数据相比好相关,结果通过其他诊断方法获得的结果和疾病的临床表现。 UFA已被证明比酶免疫测定更敏感。

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