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首页> 外文期刊>BioMed research international >Hepatoprotective and Antioxidant Effects of Saponarin, Isolated from Gypsophila trichotoma Wend. on Paracetamol-Induced Liver Damage in Rats
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Hepatoprotective and Antioxidant Effects of Saponarin, Isolated from Gypsophila trichotoma Wend. on Paracetamol-Induced Liver Damage in Rats

机译:从满天星满天星分离的皂苷的保肝和抗氧化作用。扑热息痛对大鼠肝损伤的影响

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The hepatoprotective potential of saponarin, isolated from Gypsophila trichotoma, was evaluated in vitro/in vivo using a hepatotoxicity model of paracetamol-induced liver injury. In freshly isolated rat hepatocytes, paracetamol (100 mumol) led to a significant decrease in cell viability, increased LDH leakage, decreased levels of cellular GSH, and elevated MDA quantity. Saponarin (60-0.006 fig/mL) preincubation, however, significantly ameliorated paracetamol-induced hepatotoxicity in a concentration-dependent manner. The beneficial effect of saponarin was also observed in vivo. Rats were challenged with paracetamol alone (600 mg/kg, i.p.) and after 7-day pretreatment with saponarin (80 mg/kg, oral gavage). Paracetamol toxicity was evidenced by increase in MDA quantity and decrease in cell GSH levels and antioxidant defence system. No changes in phase I enzyme activities of AH and EMND and cytochrome P 450 quantity were detected. Saponarin pretreatment resulted in significant increase in cell antioxidant defence system and GSH levels and decrease in lipid peroxidation. The biochemical changes are in good correlation with the histopathological data. Protective activity of saponarin was similar to the activity of positive control silymarin. On the basis of these results, it can be concluded that saponarin exerts antioxidant and hepatoprotective activity against paracetamol liver injury in vitro/in vivo.
机译:使用对乙酰氨基酚诱导的肝损伤的肝毒性模型在体外/体内评估了从毛满天冬满天星分离的皂苷的保肝潜力。在新鲜分离的大鼠肝细胞中,扑热息痛(100μmol)导致细胞活力显着下降,LDH渗漏增加,细胞GSH水平下降和MDA含量升高。然而,皂苷(60-0.006 fig / mL)的预孵育以浓度依赖的方式显着改善了扑热息痛引起的肝毒性。在体内也观察到了皂苷的有益作用。单独用扑热息痛(600 mg / kg,腹腔注射)攻击大鼠,然后用皂苷(80 mg / kg,口服管饲)预处理7天。扑热息痛的毒性通过MDA量的增加和细胞GSH水平的降低以及抗氧化防御系统来证明。没有检测到AH和EMND的I期酶活性和细胞色素P 450量的变化。皂苷预处理可导致细胞抗氧化防御系统和GSH含量显着增加,脂质过氧化作用降低。生化变化与组织病理学数据高度相关。皂苷的保护活性类似于阳性对照水飞蓟素的活性。基于这些结果,可以得出结论,皂苷在体内/体外对扑热息痛的肝损伤具有抗氧化和保肝活性。

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