Production of transgenic rapeseed Brassica napus L. by transformation with Agrobacterium tumefaciens

摘要

The procedure for genetic transformation of two spring an done winter rapeseed cultivars was developed. Nopaline strains of Agrobacterium tumefaciens GV3101 and EHA105 were shown to be preferable for gene transfer, as compared to the octopine strain GV2260. With two types of plant explants,t he segments of hypocotyls and cotyledons, transformation was successful: however, its efficiency was somewhat higher with the fragments of hypocotyls. Analysis of regenerated plants by PCR and southern blotting confirmed the presence of the nptII and nisA genes in transformants. RNA analysis by Northern blotting showed expression of the nisA gene in transformed shoots. The transgenes were inherited in T_2 as Mendelian traits. The effect of biotic and abiotic factors in the efficiency of genetic transformation in rapeseed is rapeseed is discussed.