Enzymatic synthesis of tritium-labeled #beta#-NAD~+ and its use in the poly(ADP-ribose) polymerase assay

S. I. Shram; I. G. Rybakova; T. Yu. Lazurkina

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Enzymatic synthesis of tritium-labeled #beta#-NAD~+ and its use in the poly(ADP-ribose) polymerase assay

机译：氚标记的氚标记的β-β＃-NAD〜+及其在聚（ADP-核糖）聚合酶测定中的用途

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摘要

A method of preparation of tritiated #beta#-NAD~+ from (adenine-2,8-~3H)ATP and nicotinamide mononucleotide is suggested. This method is based on the baker's yeast (Saccharomyces cerevisiae) enzyme nicotinamide nucleotide adenylyltransferase. Experimental conditions for a nearly complete conversion of labeled ATP to NAD were determined. The tritiated NAD prepared by this method can be used in the quantitative assay of the chromatin enzyme poly(ADP-ribose) polymerase.

机译：提出了一种制备氚β-NAD〜+的方法（腺嘌呤-2,8-〜3H）ATP和烟酰胺单核苷酸。该方法基于面包师的酵母（酿酒酵母酿酒酵母）酶烟酰胺核苷酸腺苷酰亚胺酰基转移酶。确定了标记为ATP与NAD的几乎完全转化的实验条件。通过该方法制备的氚化NAD可用于染色质酶聚（ADP-核糖）聚合酶的定量测定。

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来源
《Прикладная биохимия и микробиология》 |1999年第6期|共9页
作者
S. I. Shram; I. G. Rybakova; T. Yu. Lazurkina;
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作者单位

Institute of Molecular Genetics Russian Academy of Sciences Moscow 123182 Russia;

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原文格式 PDF
正文语种 rus
中图分类生物化学;
关键词
enzymatic synthesis; tritium-labeled; beta; -NAD~+; poly(ADP-ribose) polymerase assay;

机译：酶合成;氚标记;β;- NAD〜+;聚（ADP-核糖）聚合酶测定;

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1. Enzymatic synthesis of tritium-labeled #beta#-NAD~+ and its use in the poly(ADP-ribose) polymerase assay [J] . S. I. Shram, I. G. Rybakova, T. Yu. Lazurkina Прикладная биохимия и микробиология . 1999,第6期

机译：tri标记的＃beta＃-NAD〜+的酶促合成及其在聚（ADP-核糖）聚合酶测定中的应用
2. Enzymatic synthesis of tritium-labeled #beta#-NAD~+ and its use in the poly(ADP-ribose) polymerase assay [J] . S. I. Shram, I. G. Rybakova, T. Yu. Lazurkina Прикладная биохимия и микробиология . 1999,第6期

机译：氚标记的氚标记的β-β＃-NAD〜+及其在聚（ADP-核糖）聚合酶测定中的用途
3. Enzymatic synthesis of tritium-labeled beta-NAD and its use in the poly (ADP-ribose) polymerase assay [J] . Shram S.I., Rybakova I.G., Lazurkina T.Yu, Applied biochemistry and microbiology . 1999,第6期

机译：tri标记的β-NAD的酶促合成及其在聚（ADP-核糖）聚合酶测定中的应用
4. DESIGN AND SYNTHESIS OF NOVEL POLY ADP-RIBOSE POLYMERASE 1(PARP1)INHIBITORS FOR THE TREATMENT OF SOLID TUMORS [C] . J.Priyancy, D.P.Bhumika Conference on Drug Design and Discovery Technologies . 2020

机译：用于治疗实体肿瘤的新型聚ADP-核糖聚合酶1（PARP1）抑制剂的设计与合成
5. Design and Synthesis of Poly(ADP-ribose) Polymerase Inhibitors: Impact of Adenosine Pocket-Binding Motif Appendage to the 3-oxo-2,3-dihydrobenzofuran-7-carboxamide on Potency and Selectivity [D] . Velagapudi, Uday Kiran. 2019

机译：聚（ADP-核糖）聚合酶抑制剂的设计与合成：腺苷袋结合基序附录对3-氧代-2,3-二氢苯并呋喃-7-甲酰胺对效力和选择性的影响
6. A Quantitative Assay Reveals Ligand Specificity of the DNA Scaffold Repair Protein XRCC1 and Efficient Disassembly of Complexes of XRCC1 and the Poly(ADP-ribose) Polymerase 1 by Poly(ADP-ribose) Glycohydrolase [O] . In-Kwon Kim, Roderick A. Stegeman, Chris A. Brosey, 2015

机译：定量分析揭示了DNA支架修复蛋白XRCC1的配体特异性以及XRCC1和聚（ADP-核糖）聚合酶1的复合物通过聚（ADP-核糖）糖基水解酶的有效拆卸。
7. The Development of a Biotinylated NAD+-Applied Human Poly(ADP-Ribose) Polymerase 3 (PARP3) Enzymatic Assay [O] . Ming Ji, Liyuan Wang, Nina Xue, 2018

机译：生物素化的NAD + -Applied人聚（ADP-核糖）聚合酶3（PARP3）酶测定的研制

Enzymatic synthesis of tritium-labeled #beta#-NAD~+ and its use in the poly(ADP-ribose) polymerase assay

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