Novel amperometric glucose biosensor based on covalent immobilization of glucose oxidase on poly(pyrrole propylic acid)/Au nanocomposite

摘要

A glucose biosensor is fabricated with immobilization of glucose oxidase (GOx) on poly(pyrrole propylic acid)/Au nanocomposite by covalent attachment. Poly(pyrrole propylic acid) (PPyAA)/Au nanocomposite was prepared by chemical oxidation of pyrrole propylic acid monomer by using chloroauric acid (HAuCl _4) as an oxidizing agent. The obtained nanocomposites were used to fabricate highly sensitive amperometric glucose biosensor which exhibited a high and reproducible sensitivity of 0.42 μA/mM, response time ～2 s, linear dynamic range from 1 to 18 mM, correlation coefficient of R ~2 = 0.9981, and limit of detection (LOD), based on S/N ratio (S/N = 3) of 0.05 mM. A value of 1.83 mM for the apparent Michaelis-Menten constant Kmapp was obtained. The high sensitivity, wider linear range, good reproducibility and stability make this biosensor a promising candidate for portable amperometric glucose biosensor.