Respiratory Activity of the Bacterium Acinetobacter calcoaceticus TM-31 during Assimilation of Alkane Hydrocarbons

摘要

the respiratory activity of Acinetobacter calcoaceticus TM-31 with resect to alkane hydrocarbons was studied, The dynamics of oxygen consumption by the cells while assimilating n-hexadecane was assayed by a modified technique using an oxygen electrode. The dependence of cell respiratory activity on the amount of n-hexadecane within the concentration range of 0.13-0.66% was determined. It was demonstrated that the cells also displayed respiratory activity towards other medium-chain n-alkanes: hexane, octane, decane, tridecane, and heptadecane. Thus, we demonstrated the possibility of determining alkanes by measuring the respiratory activities of microorganisms.