ACUTE EFFECT OF HH2O ON OXYGEN CONSUMPTION RATE,INTRACELLULAR ATP AND ROS IN FRESHLY ISOLATED HUMAN PERIPHERAL BLOOD MONONUCLEAR CELLS

摘要

Human peripheral blood mononuclear cells (PBMC) were used as a biological model to measure intracellular ATP (iATP) reactive oxygen species (ROS), Oxygen Consumption Rate (OCR) and extracellular acidification rate (ECAR), in response to acute ex vivo treatments with HH2o. "HH2o ", a fermented, food-based material commercially marketed as "Mitochroma~(TM)" has been identified as a potential modulator of iATP, OCR and ROS in these cells. PBMC were exposed to HH2o (0.1, 0.01 and 0.001 % w/v) and Carbonyl-cyanide p-trifluoro-methoxy-phenyl-hydrazone (FCCP) or Oligomycin, two known modulators of mitochondrial activity were used as controls. Results showed that treatment of the cells with HH20 resulted in increase of iATP up to 220% and reduction of iADP by up to 40% while iROS levels remained unchanged. At the same time, OCR level was increased up to 24% compared to initial baseline. These results suggest that HH2o acts similarly to uncoupling protein 2 (UCP2) when induced and that it may have potential use in developing health-promoting products. These results support the use of freshly isolated human PBMCs as an experimental ex vivo model to detect the effects of nutraceutical products on dynamic cell metabolites, such as oxygen consumption, A TP, ADP and ROS.