首页> 外文期刊>Current topics in nutraceutical research >RANDOM-CENTROID OPTIMIZATION OF ULTRASOUND-ASSISTED EXTRACTION OF POLYSACCHARIDES FROM CORDYCEPS SINENSIS MYCELIUM
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RANDOM-CENTROID OPTIMIZATION OF ULTRASOUND-ASSISTED EXTRACTION OF POLYSACCHARIDES FROM CORDYCEPS SINENSIS MYCELIUM

机译:超声波辅助提取冬虫夏草多糖中多糖的随机重心优化

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摘要

Ultrasound-assisted extraction was used to extract crude polysaccharides (PS) from Cordyceps sinensis (Cs-4) mycelium, and the effects of processing parameters on polysaccharide yields were optimized by random-centroid optimization (RCO). The optimal extraction conditions were as follows: liquid to solid ratio of 30 mL/g, ultrasonic power of 550 W ultrasonic time of 20 min, and extraction time of 3. Under these conditions, the maximum extraction yield of PS was 1660%. The partially purified PS-1 contained high-molecular weight (730 kDa, 80.86%) and low-molecular-weight (5.8 kDa, 19.14%) polysaccharides composed of D-glucose, D-mannose, and D-galactose in a ratio of 1.8:1.2:1.0 with cc-type glycosidic bonds. The results indicated that ultrasound-assisted extraction was a very powerful and efficient tool for the extraction of polysaccharides from fungi or mushrooms, and RCO was an useful approach for experimental variable optimization.
机译:采用超声辅助提取法从冬虫夏草(Cs-4)菌丝体中提取粗多糖(PS),并通过随机质心优化(RCO)优化了工艺参数对多糖产量的影响。最佳提取条件为:固液比为30 mL / g,超声功率为550 W,超声时间为20 min,提取时间为3。在这些条件下,PS的最大提取率为1660%。部分纯化的PS-1含有高分子量(730 kDa,80.86%)和低分子量(5.8 kDa,19.14%)的多糖,其比例为D-葡萄糖,D-甘露糖和D-半乳糖。 1.8:1.2:1.0具有cc型糖苷键。结果表明,超声辅助提取是从真菌或蘑菇中提取多糖的非常有效的工具,RCO是优化实验变量的有用方法。

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