Expression of the cercosporin transporter,CFP,in tobacco reduces frog-eye lesion size

摘要

The cercosporin Major Facilitator Superfamily (MFS) transporter,CFP,under the control of the CaMV 35S promoter,was introduced into the Xanthi cultivar of tobacco by Agrobacterium-mediated transformation.CFP~+ transgenic plants were physically indistinguishable from non-transgenic Xanthi and progressed normally through growth to seed set.Accumulation of CFP in the leaf membrane fraction of CFP ~+ transgenic plants was associated with decreased cercosporin phytotoxicity.Frog-eye leaf lesions on CFP~+ transgenic plants infected with Cercospora nicotianae conidia were smaller but were similar in number to those on non-transgenic plants.We conclude that transgenic expression of CFP may have relevance for a disease control strategy in Cercospora-plant pathosystems where cercosporin is implicated in pathogen virulence.