Development of a biosensor for measuring plasma fibrinogen based on an enzyme kinetic model

摘要

A biosensor has been designed for measuring plasma fibrinogen concentrations based on an enzyme kinetic model.The values obtained correspond linearly to those of a clinical method but have a higher sensitivity within the normal range of fibrinogen.By using the disposable biosensor technology,this method is easy to follow,quick (within 2 min) and precise (with coefficients of variation to be as low as below 5%) and requires only a small amount of plasma sample (<50 mul).This biosensor technology also holds potential for use in point-of-care diagnostics.