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首页> 外文期刊>Biotechnology Letters >Real Time RT-PCR and flow cytometry to investigate wheat kernel hardness:role of puroindoline genes and proteins
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Real Time RT-PCR and flow cytometry to investigate wheat kernel hardness:role of puroindoline genes and proteins

机译:实时RT-PCR和流式细胞仪研究小麦籽粒硬度:嘌呤二氢吲哚基因和蛋白质的作用

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摘要

Developing seeds from Triticum aestivum(wheat)cultivars were collected after flowering and analysed for puroindoline a and b gene expression by Real Time RT-PCR.Mature seeds were investigated for the presence and the amount of starch-associated puroindoline a and b proteins by flow cytometry.Puroindoline a gene and protein were found to have a predominant role in controlling wheat kernel hardness.
机译:开花后从小麦(小麦)栽培品种中收集发育中的种子,并通过实时RT-PCR分析嘌呤二氢吲哚a和b基因的表达,并通过流式分析成熟种子中淀粉相关的嘌呤二氢吲哚a和b蛋白的存在和数量。发现Puroindoline a基因和蛋白质在控制小麦籽粒硬度中起主要作用。

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