Functiional analysis of the blood brain barrier using conditionally immortalized cell lines.

摘要

In vitro cell culture system is one of useful tools for analyzing function of the blood brain barrier(BBB).We have established conditionally immortalized endothelial cell lines from the transgenic rat and mouse harboring temperature sensitive SV 40 large T antigen gene.These culture cells expressed GLUT-1 and mdr 1a and their growth depended on the expression of ts SV40T antigen in cells.Using these cell lines together with in vivo analyzing system such as brain efflux index method,we revealed following new BBB functions:1.Amino acid transport involved in osmoregulation.2.Stereoselective acidic amino acid transport.3.Efflux transport system of neurotransmitter,GABA.4.Efflux transport system of neurotrasmitter metabolites,homovanillc acid,and effect of uremic toxin on the efflux system.5.supply creating as energy depot to brain.These evidences suggested that the BBB is not only a supporting system but also a protecting system for amintaining the central nevvous ststem.