Interaction of endogenous compounds in human and rat urine with p-glycoprotein

摘要

The effect of hydrophobic extracts of human and rat urine on in-vitro P-glycoprotein (P-gp) function was examined,in terms of intra-,inter-individual variations,and physiological states.Six urine samples out of 7,obtained fromone male subject ondifferent days,suppressed P-gp function with different potencies.Similarly,11 samples out of 15 different individuals (8 males and 7 females) inhibited P-gp function.Among them,urine fromone female,obtained 1 month after delivery,showed apotent inhibitory effect.Another urine froma pregnant female,obtained 1 week before delivery,showed further potent inhibition on P-gp function.Inaddition,urine fromnormal rats strongly inhibited P-gp function at much lower concentrations than human urine,and the inhibitory potencies variedin diseased states;control (without urine extract)=experimental acute renal failure