Cloning and functional analysis of a novel chitinase gene Trchil from Trichoihecium roseum

摘要

Chitinases produced by mycoparasites play an important role in disease control in plants. To explore the functions of chitinases in Trichoihecium roseum, we cloned a new chitinase gene named Trchil from T roseum by RT (reverse transcription)-PCR techniques. The T. roseum gene, Trchil, contains an 1278-bp ORF that shares 76 % similarity with chitinase from Bionectria ochroleuca (ABV57861 3G6L_A). A plant expression vector, containing the Trchil gene driven by the CaMV35S promoter, was constructed andtransformed into tobacco via Agro-bacterium tumefaciens. Southern blot analysis showed that Trchil was integrated into the tobacco genome.