Highly Fluorescent Amidine/Schiff Base Dual-Modified Polyacrylonitrile Nanoparticles for Selective and Sensitive Detection of Copper Ions in Living Cells

摘要

Highly fluorescent surface modified polyacrylonitrile nanoparticles (PAN NPs) of 50 nm diameter were fabricated for selective Cu~(2+) sensing. After surface modification, the PAN NPs were converted to amidine/SchifF base dual-modified PAN nanoparticles (tPAN NPs) with a Cu~(2+) sensing property and high QY (0.19). The selectivity of tPAN NPs for Cu~(2+) is much higher than that of other metal ions due to the fact that amidine group on the surface of tPAN NPs has a higher binding affinity with Cu~(2+). The effect of other metal ions on the fluorescence intensity of the tPAN NPs was also studied, and other metal ions showed a low interference response in the detection of Cu~(2+). Furthermore, as a metal ion chelator, ethylenediaminetetraacetate can competitively interact with Cu~(2+) to recover the quenched fluorescence of tPAN NPs. The tPAN NPs are easily introduced into cells and exhibit low toxicity, enabling their use as a fluorescence sensor for Cu~(2+) in living cells. The tPAN NPs provide a new direction for the development of copper ion sensors in living cells.