Preparation of highly unsaturated fatty acid-containingphosphatidylcholine by transesterification with phospholipase A(2)

摘要

To prepare phospholipids containing highly unsaturated fatty acids (HUFA) by a one-step enzymatic reaction, 14 mu mol soy phosphatidylcholine (soy PC) in combination with 60 mu mol HUFA as acyl donor were transesterified with porcine pancreatic phospholipase A(2). Specify HUFA was successively incorporated into the sn-2 position of soy PC by this reaction at greater than 70% of total fatty acids at sn-2.