Lipophorin uptake into developing ovariole in Bombyx mori (Bombycidae: Lepidoptera)

摘要

Carbohydrates, proteins and lipids play an important role in the overall physiology of insects. Here, we describe the lipid distribution in internal organs by using fluorescence lipid, which shows that more lipids were accumulated in the ovary than in the fat body and cuticle. On the other hand, protein accumulation in pupal developing ovary was resolved by SDS-PAGE, showing that vital proteins like vitellin, 30 kDa, egg specific protein and lipophorin were gradually accumulated in the ovary. Furthermore, well-differentiated ovariole was observed in day 4 pupal ovary, and we examined the lipophorin uptake by using the fluorescence immunolocalization technique. Lipophorin was present in nurse cells and follicle cells and marginally with perioocyte space in immature ovariole. In the mature ovariole, nurse cell was narcotized and lipophorin appeared in follicular cell. Altogether, present findings illustrate that lipids and proteins gradually accumulated in the developing ovary. But lipophorin was endocytic uptake in immature ovariole, after the maturation uptake halted.